РАЗРАБОТКА РЕЦЕПТУРЫ И ТЕХНОЛОГИИ ПЕНООБРАЗОВАТЕЛЯ ИЗ МАСТИЧНОЙ (PISTACIA LENTISCUS L.) КАМЕДИ.

An acid fraction derived from the evergreen plant of Mastic gum(Pistacia lentiscus L., family Anacardiacae, genus Pistacis L.) has been found to have bactericidal activity against 11 strains of Helicobacter pylori. However its healing potential is significantly reduced by poor solubility and low biological penetration. Therefore, the current problem is to develop a system that provides increased penetration of the drug into the stomach through the epithelial barrier. Foams are especially interesting in this regard. Foams are light systems, they do not swell, on the contrary, they grow in volume, completely covering the mucous membrane. The foams do not require taste correction and are designed to deliver a therapeutic substance from the skin and mucous membranes and provide an effective treatment. The aim of the research was the determination of formulation and development of technology of innovative medicinal form from Mastic gum. Based on biopharmaceutical studies, the formulation of capsules containing a foaming powder composition was determined: Mastic gum 250.0; Sodium lauryl sulfate (SLS) - 9.0; Lecithin - 12.6; Xanthan 4.5; Gelatin 4.5; Isomalt 90.0; Dry egg whites - 41.0; Sodium bicarbonate - 21.0; Citric acid - 6.0; Metocell M102 - 10.0; Talc - 1,4. The technology of preparation of Mastic gum foaming powder containing capsules has been developed.Foaming capsules containing Mastic gum, in artificial gastric juice, with foam-forming ability and foam stability meet the standard requirements for foam systems.

[STUDY OF THE CYTOTOXIC ACTIVITY OF INDOLE ALKALOIDS FROM THE ABOVEGROUND ORGANS OF VINCA ROSEA L. INTRODUCED IN WESTERN GEORGIA].

The objects of this research were the aboveground and underground parts of Vinca rosea L., a species of periwinkle introduced in Western Georgia. The sum of alkaloids and biologically aactive fractions of monomeric alkaloids were obtained by liquid-liquid extraction, precipitation with petroleum ether (I), polybuffer distribution (II) and 10% acetic acid (III). 14 known compounds were identified by LC-MS/MS and GC/MS: Low molecular weight compounds (М+226, М+202, №1,2, М+168) and alkaloids Ajmalicine, Tetragidroalstonine, C20-dihydrova llesiahotamine, C19-C20 Vallesiahotaminole, Vallesiahotamine lacton, Polyneuridine, Pericy clivine, Lochnerame, Norharmane, Vidorosine, Vindolinine, Isovindolinine, Akuammicine. The cytotoxicity of monomeric alkaloids was evaluated for A-549 (cells of the lung cancer line), DLD-1 (cells of the rectal adenocarcinoma line) and W-1 (cells of the normal human fibroblast line). All three substances (I, II, III) showed pronounced cytotoxic activity.

STUDY OF THE BIOACTIVE COMPOUNDS CONTENT IN THE FLOWERS OF POLIANTHES TUBEROSA L. INTRODUCED BY GREEN TECHNOLOGIES.

The content of bioactive compounds of perennial, fragrant species of tuberose or Polianthes tuberosa L. representing the genus Polianthes L., asparagus (Asparagaceae Juss) family, highly decorative and widely accepted in the perfumery industry and extremely sensitive to environmental conditions, native to Mexico, was studied based on of raw materials of the flowers of the said plant introduced to five different locations of the BBG with various exposition and soil content by green technologies. Based on the analysis of the soil obtained from all five locations, we can conclude, that the growth and development of the plant along with the quality and amount of received raw materials significantly depend on the rate of soil acidity, humus, and the content of basic feeders. Good results were revealed in the conditions of the experimental plot of fragrant plants with well enough warmth, sunlight, and humidity. Applying 100% natural bio preparation GeoHumate had a positive influence on the plants on the experimental plot in terms of the growth and development of the plant and accumulation of bioactive compounds. After doing GC-MS researches of tuberose, Polianthes tuberosa L., flowers introduced by green technologies in five different locations of the BBG with various exposition and soil content, there were identified important compounds valuable for cosmetics, perfumery, medicine, and of different fields.

ANTIHYPERTENSIVE AND CARDIOPROTECTIVE EFFECTS OF EPOXYEICOSATRIENOIC ACID ANALOGS AND SOLUBLE EPOXIDE HYDROLASE INHIBITORS (REVIEW).

In the present article is considered antihypertensive and cardioprotective action of epoxieicosatrienoic acids (EETs) analogues and soluble epoxide hydrolase (sEH) inhibitors. Being epoxygenase products of arachidonic acid metabolism EETs provide a wide spectrum of biological activity in different organs implicated in the regulation of arterial pressure, including vascular endothelium (VE), heart and kidney. EETs are acting as autocrine or paracrine agents producing vasodilation, reduction in salt and fluid retention. EETs exert vasodilatory properties by activation of the smooth muscle large conductance Ca2+ -activated K+ channels in different vascular beds. In experimental and clinical studies EETs provide beneficial influence in hypertensive states alleviating vascular endothelium function associated with reduction of inflammation and increased Na+ excretion, prevented cardiovascular and renal complications accompanied arterial hypertension (AH). In animals studies using Dahl-salt-sensitive (Dss) hypertensive rats EETs analogues displayed renoprotective effect, reducing mRNA expression of tumor growth factor-"B" as well as concomitant oxidative stress and fibrotic changes in the heart and kidneys. In cultered endothelial cells also was demonstrated anti-inflammatory action of EETs when they significantly decreased TNF-α-induced high level of monocyte chemoattractant protein-1, which was reversed by EETs antagonist. sEH is another new target for therapeutic intervention in cardiovascular diseases. In several experimental models of AH sEH inhibitors (sEHI) significantly reduce AP which was associated with their anti-inflammatory and renoprotective action. sEHI showed cardiovascular effect related to CYP2J2overexpression during myocardial ischemia-reperfusion injury. It should be noted that EETs demonstrate binding ability to PPAR receptor "Y" stimulating it's transcription process in endothelial cells facilitating by sEHI. It is suggested that EETs and sEH are involved in the regulation of the cardiovascular function playing significant role in vascular homeostasis.

FORMULATION THERMORESPONSIVE NANOCOMPOSITE HYDROGELWITH EMBEDDED PLGA NANOPARTICLES CONTAINING CYTOTOXIC AGENT.

The aim of the study was to develop and characterize the nanocomposite in-situ hydrogel as local drug delivery system of cytotoxic agent. In-situ hydrogel consisting of 15% thermosensitive (Poloxamer 407) and 1% mucoadhesive (sodium alginate) polymers was selected as the optimal formulation by the conducted studies. The influence of nanoparticle concentration on gelation time and temperature has been experimentally established. As a result, the optimum concentration of nanoparticles (5%) is selected, which does not alter the gel forming process. The resulting nanocomposite hydrogel was characterized through Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rotational viscometer (LVDV-1T). FT-IR spectra confirmed the PLGA nanoparticles presence within the hydrogel matrix through the absorption peak located at 1750 cm-1. SEM images allowed observing the nanoparticles to be homogenously dispersed. The release pattern of the active substance from the nanocomposite hydrogel is following: at 72 h, 64% and 78% of the active substance were released into the phosphate buffer and cell culture area, respectively. Irritation test on hen's egg model revealed that formulated nanocomposite hydrogel did not show damage of vascular system.

BIOPHARMACEUTICAL UNDERSTANDING OF FORMULATION PREPARATION VARIABILITY OF PLGA NANOPARTICLES LOADED WITH ERYSIMUM EXTRACT.

The purpose of this study was to evaluate effect of process and formulation variables on the preparation of Erysimum extract loaded PLGA nanoparticles. The influence of the various biopharmaceutical factors such as type of organic solvent, type and concentration of surfactant, polymer concentration in the organic phase, ratio of organic phase and water phase were studied. Modified emulsification solvent evaporation method was used for preparation of nanoparticles. Based on the performed experiments optimal formulation of nanocomposite is suggested. Nanoparticle size, size distribution and entrapment efficiency were determined. Among five non-ionic surfactants polyvinyl alcohol provided more stable nanocomposite. Influence mechanisms of different surfactants on nanoparticle formation are provided. Water miscible organic solvent, acetone obtained 232 nm nanoparticles with improved size distribution. Entrapment efficiency was increased to 73% by reducing ratio of organic and water phases. Based on experiments nanoparticles with stable, reproducible properties are fabricated.

; INTRODUCTION OF AROMATIC PLANTS IN THE BATUMI BOTANICAL GARDEN AND THEIR RESEARCH FOR THE CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS.

The research objectives were to study the introduction process of different aromatic and spice plants in the Batumi Botanical Garden and the content of bioactive compounds in aromatic plants introduced and grown in the soil and climatic conditions of the garden.; Among the aromatic plants introduced to the BBG by the authors, the following species are having full vegetative and generative development in the open ground:Cuminum cyminum L.; Polianthes tuberosa L.; Iris pallida Lam.;Geranium macrorrhizum L., Piper suaveolens Ham., Piper piperita L., Thymus citriodorus Schreb., Satureja Montana L., Mentha piperita L., Origanum vulgare L., Mentha longifolia L., Hyssopus officinalis L.; Phyla scaberrima Moldenke, Ruta graveolens L.; Vegetative organs reach the full development, but without blooming:Curcuma longa L..; Zingiber officinale Roscoe.; Elettaria cardamomum Maton.; Coffea arabica L.; Coffea conephera L..; Vanilla planifolia Jacks.; Cassia acutifolia Delile.; For the purpose of studying the content of bioactive compounds in plants introduced to the soil and climatic conditions of the BBG with the help of green technologies, the following species were taken for analysis:Cassia acutifolia Delile. - seed, flower, leaf; Cuminum cyminum L. - seed; Coffea conefera L. andCoffea Arabica L., leaves and seeds; Vanilla planifolia Jacks. - leaves; Elletaria cardamomum Maton. -Cardamom leaves.With the help of gas chromatography - mass spectrometry GC-MS method, there were identified bioactive compounds of various classes. The content of essential oils is identified in all research objects.

FORMULATION OF BIODEGRADABLE POLYMERIC NANOPARTICLES CONTAINING CYTOTOXIC SUBSTANCE OF PLANT ORIGIN.

Formulation of novel drug delivery system is one of the approaches for improvement of pharmacological activity of drugs. This implies encapsulation of the API into the biocompatible polymeric material. Objective of the research was formulation of biodegradable amino acid based polyesteramide nanoparticles composing cytotoxic substance of plant origin. Research materials and methods: biodegradable polyesteramide (PEA), alkaloids from Vinca Minor, surfactants (Tween 80, polyvinyl pirolidone, polyvinyl alcohol, Poloxamer 188). NPs size (mean particle diameter) and size distribution (polydispersity index, PDI), and zeta-potential (ZP) were measured by dynamic light scattering (DLS) using a Zetasizer Nano ZS (Malvern Instruments, U.K.) at 25°C, UV spectrophotometer was used for %EE study. Amino acid based PEA particles were fabricated by the modified emulsification method. Based on the studies optimal composition and fabrication condition of PEA NPs was determined. The conditions of the NPs fabrication were as follows: the O/W ratio: 1:10; the solvent: DMSO; polymer concentration in the organic phase: 50.0 mg/mL; surfactants (PVA) concentration in aqueous phase 0.5%,the stirring rate: 1000 rpm. The influence of the various factors such as organic solvents, surfactants, as well as a polymer concentration in the organic phase,surfactant concentration in the aqueous phase,the organic/water phase ratio on the NPs fabrication was studied.The NPs were characterized by size (mean particle diameter & size distribution (polydispersity index, PDI), and zeta-potential (ZP). Increase concentration of the surfactant (polyvinyl alcohol) from 0.1% to 0.5% decrease average particle size from 568±63 to 169±1.66 respectively. EE% was obtained to be around 50%.

STUDY OF PHYSICAL AND TECHNOLOGICAL FEATURES AND CHEMICAL COMPOSITION OF CLAYS PREVALENT IN ADJARA REGION.

The Region of Adjara is one of the most interesting region by existence of mineral resources, which is not only untapped also not studied. At this stage of study, our purpose was: to research physical features and a chemical composition of biologically active substances in the clays spread in Adjara. In particular, we determined the fractional content and particle size of samples, density, humidity, fluidity, Petrographic studies and X-ray diffraction, also existence of amino and fatty acids. X-ray diffraction and petrographic studies were conducted on the base of Mining Geology Faculty of Georgian Technical University. There was used the polarization microscope Amscope PZ300T-5M and DRON-3. Determination of existence amino and fatty acids has been undertaken on the base of Tbilisi State Medical University, the method was chromatography on the thin layer. Consequently, from studying physical features we determined that mostly fractional content of our samples are from 0.5 to 1 mm. particle size. Humidity was 15%, according to density and fluidity our samples belong to middle heaviness 1100>p>600kg/m3 and high friable powders. As for X-ray diffraction and petrographic studies, there was discovered significant quantity of minerals: Ca-montmorillonite, Ca-Na feldspar, quartz, calcite, dolomite, gipsy and hematite. Based on the research there was not discovered amino and fatty acids in the clay samples.

EXTRACTION AND CHARACTERIZATION OF HUMIC SUBSTANCES FROM SPHAGNUM PEAT PELOIDS.

The objective of the research was development extraction process of humic substances from sphagnum peat peloids, selection of extragent and characterization of humic substances. The objects of the research: Kolkheti peat peloids (Ispani, Anaklia, Churia, Chirukhi, Peranga) of different formation phases. Research was held using modern instrumental methods of analysis: UV spectrophotometer, Scanning Electron Microscopy, Centrifuge, Dry oven, Ultraturax. In the research extraction process of humic substances from sphagnum peat peloids was developed and composition of humic substances was studied, also E4/E6 humification coefficient was evaluated. Based on the results extraction conditions of humic substances from the peat peloids samples were determined: a) extragent with maximum yield - 1.0N NaOH; b) mixing type - KA-ULTRA TURAX-T18 - 20 000 rpm/min; c) Precipitant of humic substances -10% HCL. Composition of humic substances are studied in the samples and their relatively high content is determined in Anaklia and Churia sphagnum peat peloids. For characterization of humic substances E4/E6 humification coefficient was evaluated. Low ration of E4/E6 < 5 was established in anaklia, churia and ispani peat peloids. High ration of E4/E6 < 10 coefficient is determined in chirukhi and peranga peat peloids.

DEVELOPMENT OF THE FORMULATION AND TECHNOLOGY OF HYDROGEL, CONTAINING ADJARA REGION SULFIDE SILT PELOID.

In the world today, the demand for the preparations and cosmetics, made of peloids is increasing significantly, which is explained by the increased interest of the community to the ecologically clean raw materials of natural origin, it often replaces expensive chemical preparations, which are often accompanied by some contraindications. The increased interest in peloids in the world put on the agenda the question of rational use of acting mud mines as well as the problem of cosmetic and medicinal preparations, developed on their basis. It is noteworthy that the development of ready-made, soft drug forms from the Adjara region sulfide silt peloids is one of the most topical and important issues, as at present there is not available the form of ready-made drugs containing the sulfide silt peloids of this region. Therefore, the aim of the research was to develop the formulation and technology of the hydrogel, containing the Adjara region sulfide silt peloid. The sulfide silt peloid of the Ardagani lake have been used for preparing the hydrogel compositions, as it is distinguished by its advantageous physical-chemical properties and content of biologically active ingredients compared with other studied peloids. The main physical-chemical and technological characteristics of hydrogels compositions have been studied using the following methods: uniformity, pH, the colloidal stability, thermal stability , osmotic activity - by dialysis method using semiconductor membrane (Kruvichinski method), release of active ingredient was studied by using Franz diffusion cells, viscosity was determined by Viscometer RVDV-1 T (Dongguan Zhongli Instrument Technology Co., China), the structure of hydrogel composition - by microscopic (ROW Rathenow, Germany) examination. Based on the conducted studies have been established, that the hydrogel composition N6 is distinguished by its best properties compared to other hydrogel compositions, in which the carboxymethylcellulose was used as a base.The conducted studies became the basis for the development of the formula and technological scheme of production for the hydrogel of Ardagani lake sulfide silt peloid of Adjara region. The results of determining the main characteristics of the given gel provide the desired quality and efficiency of the product.

CHARACTERIZATION OF SULPHIDE SILT PE-LOIDS IN SOME LAKES WITHIN ADJARA REGION AND DETERMINATION OF THE BIOLOGICALLY ACTIVE COMPOUNDS CONTENT.

Medicinal muds, so called Peloids in the last decade attract attention in many countries as effective means for the treatment- preventive, and perfumery-cosmetic purposes. It has been established that the possibilities of this formation of nature are not limited to the many-year experience of the mankind. In terms of further improvement of the human social sphere, nowadays peloids are referred to as XXI century material. The aim of the research was to study the physical-chemical characteristics, such as: humidity, pH, ash content, bulk (volumetric) density and organoleptic properties of sulphide silt peloids of some Adjara region lakes, as well as to determine the content of mineral and organic compounds (humic and organic acids). The objects of the study were the sulphide silt peloids of some Adjara region lakes: Mtsvane, Gasaliani, Shratiani, Nurigeli, Ardagani and Niphi. The main good quality indicators of the sulphide silt peloids have been determined according to the methods described in the State Pharmacopoeia XI. The mineral and organic compounds in the research objects have been determined using the modern instrumental methods of analysis described in the literature. Based on the performed studies, the main physical-chemical characteristics of sulphide silt peloids of some Adjara region lakes (Mtsvane, Gasaliani, Shratiani, Nurigeli, Ardagani and Niphi) have been studied and their mineral contents (Ca-Na Feldspar, K Feldspar, Ca- Montmorillonite, Hematite, Quartz, Magnetite, Chlorite) determined. By the spectrophotometric and gravimetric methods of analysis there is established, that the sulphide silt peloids of the lakes Ardagani (1,22%), Niphi (1,72%) and Gasaliani (1,33%) are distinguished by relatively higher content of humic acids. By the Gas Chromatography-Mass Spectrometry - method of analysis, in the study objects there is established the content of complex ethers of the following organic acids: Stearic, Palmitic, Caprylic, Myristic, Valeric, Lauric, Oxalic, Phthalic and Fumaric acids.

FORMULATION AND TECHNOLOGY DEVELOPMENT OF HERBAL PHENOLIC BIOPOLYMER-CONTAINING FILMS FOR BURN TREATMENT.

Application of phytofilms based on biosolublepolymers is considered as a prospectivemethod for burn treatment . Herbal remedies contain biologically active substances, that are relatively less toxic, do not cause skin irritation or allergic reactions and, importantly, affectstrains of the microorganisms and viruses resistant to antibiotics and synthetic drugs. Nowadays, the advantages are given to such burn healing drugs, which along with high specific efficacy, have analgesic, anti-inflammatory and antimicrobial effects, and don't irritate the tissues. The mentioned peculiarities are characteristic for a new herbal phenolic biopolymer poly[3-(3,4-dihydroxyphenyl) glyceric acid](PDGA), isolated from the roots and stems of different comfrey species . The aim of the study was the development of the formulation and technology of biosoluble films for burn treatment on the basis of PDGA. The optimal content of phytofilm for burn healing was selected on the basis of the biopharmaceutical study results. The impact of the film-former on the quality, adhesion and moisture absorption of the phytofilmhas been studied. The optimal degree of the phytofilm moisture, determining its high adhesive properties,was established. The film prepared on the basis of sodium alginate, with 30.4% humidity, demonstrated the greatest adhesion strength. After investigation of the PDGA release it was found, that the hydrophilic bases such as: sodium carboxymethyl-cellulose (69.2%) andsodium alginate (78,65%) appeared to be optimal among the others. At the same time, taking into consideration the disadvantages of sodium carboxymethyl-cellulose (tautening effect on burnt surface, relatively low stability), a film based on sodium alginate has been chosen. The manufacturing technology for obtaining PDGA-containing phytofilm by casting is proposed. Theshelf-lifeofproposedPDGA-containingphytofilmis 2 years.

[STUDY OF CYTOTOXIC ACTIVITY OF METHANOL EXTRACTS OF THE ENDEMIC PLANT OF THE GENUS ERYSIMUM OF THE ADJARIAN FLORISTIC DISTRICT OF GEORGIA].

According to the results, investigated methanolic extracts of endemic plant from Adjarian floristic region of Georgia do not have specific cytotoxicity against the cell lines of breast adenocarcinoma MCF-7 human, which is typical, e.g., for plant pigment curcumin. At the same time, the high specific cytotoxicity of 2 and 3 fractions towards keratinocytes gives reason for considering endemic plant extracts, of Adjarian floristic region of Georgia, as a potential pharmacological means for topical treatment of pathologies caused by increased proliferation of keratinocytes, such as psoriasis.

DEVELOPMENT OF FORMULATION AND TECHNOLOGY FOR THE POLY[3-(3,4-DIHYDROXYPHENYL)GLYCERIC ACID] GEL.

One of the most actual problems of pharmacy is the development of medication forms for external application with complex effects on (gel, emplastro, aerosol, etc.) skin wounds, burns and inflammatory factors. The centuries-old practice of using phyto-preparations (herbal remedies) proved that they have fewer side effects in comparison with synthetic drugs. Despite the wide application of herbal preparations, in the literature there is a little information about their application in development of wound and burn healing modern dosage forms. Among the medicinal plants with the mentioned pharmacological actions, comfrey (Symphytum L.) should be distinguished. Phenolic polymer poly[3-(3,4-dihydroxyphenyl)glyceric acid] (PDGA) or poly[oxy-1-carboxy-2-(3,4-dihydroxyphenyl)ethylene], amounting approximately 25% of polysaccharides and 1.5-2.5% of dry plant material, were isolated from the roots and stems of Caucasian comfrey species (S. asperum, S. caucasicum). Contrary to polysaccharides this phenolic polymer of Comfrey appeared to have a high immunomodulatory (anticomplement), antioxidative, antilipoperoxidantive, anti-inflammatory and wound-healing efficacy/activities. The aim of the study was development of the composition and technology of PDGA-containing gel. According to the results of complex biopharmaceutical studies PDGA gel optimal composition has been proved. The technological scheme for preparation of PDGA gel has been developed. PDGA gel stability under normal conditions of storage at +40С was studied. The gel has a shelf life (determined expiration date) of 2 year.

[COMPARATIVE ANALYSIS OF ANTIBACTERIAL EFFECT OF PHYTOANTIBIOTICS AND ANTIBIOTICS].

The objective of this study was to determine the antibacterial activity of individual medicines and containing sum of the phyto-extracts against the bacteria causing nosocomial infections in compare to antibiotics. In the investigation were involved four strains of gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Acinetobacter spp. Each object was investigated on antibiotic resistance using Kirby-Bauer diffusion method on 22 types of antibiotics. The objects of the study were: Sanguinarine, Chelerythrine and Berberine hydrochloride (of lab quality); essential oils and aromatic waters of Eucalyptus, Salvia and Lavanda. For determination their antibacterial activity was used Raits serological dilution method with adaptation. The results show that the antibiotic resistant bacteria did not rise in non-diluted and 1:1 diluted test-tubes. In 1:5 diluted test-tubes was observed a little turbidity and growth, as for in other test-tubes - intensive growth. The investigated phyto-medicines show the higher antibacterial activity than the antibiotics.

[Analysis of some species of magnolia introduced to west georgia, on content of aporphine alkaloids and their biological activity].

The goal of research was study of vegetative organs of Magnolia species introduced to west Georgia on qualitative and quantitative content of aporphine alkaloids and evaluate cytotoxic activity of total alkaloids from M. officinalis and M. glauca against A-549, DLD-1 and WS-1. Qualitative and quantitative content of aporphine alkaloids in different vegetative organs were determined by chromatographic methods. Based on the researches alkaloids - liriodenine and lanuginozine are characteristic for leaves, for bark of branches - liriodenine, d-caaverine; for bark of trunk - a liriodenine, a caaverine and a magnoflorine. Liriodenine may be considered as is chemotaxonomic sign of genus Magnolia, as it was found in all analyzed vegetative organs. In vitro cytotoxic activity of total alkaloids of M. officinalis and M. glauca has been investigated against DLD-1, WS-1 and A-549. Total alkaloids of M. glauca expressed significant cytotoxic activity against DLD-1 and WS-1, and medium cytotoxicity against A-549; M. officinalis exerted middle activity against A-549, DLD-1, WS-1.

Analysis of Perilla nankinensis decne essential oil using gas chromatography coupled with time-of-flight mass spectrometry.

Perilla is the genus of herbaceous plants of Lamiaceae family. The essential oil of Perilla is believed to be essential part of the herb. It was claimed to have anti-inflammatory, anti-hyperlipidemia, antioxidative and antimicrobial activities. Apart from pharmaceutical and nutrition purposes, It is an edible plant frequently used as a fresh vegetable. It was also applied to produce perfume and cosmetics. Due to the importance of the essential oil from the P. nanakinensis and the lack of detailed studies of them, our work aimed investigation of contents of essential compositions by GC- TOF/MS method. The materials of the study were the aerial parts of P. nankinesis collected at full flowering stage, Guria Region, Georgia. A qualitative analysis of the individual compounds in P. nankinensis essential oil performed by gas chromatography (GC) coupled with time-of-flight mass spectrometer (TOF/MS) for the identification of the resolved peaks. 28 components, which accounted for 77,7% of the oil, were identified. The main components of this essential oil were 1,3,6,10-Dodecatetraene, 3,7,11-trimethyl-, (Z,E)- (α-Farnesene) (34.3%), Caryophyllene oxide (10.2%), 1,6-Octadien-3-ol, 3,7-dimethyl- (linalool) (10.2%). Essential oil also contained humulene (3.9%),caryophyllene (1.9%), methyl salicilate (1.15%), 2-hexanoylfuran (1.0%), gernacrene (1.1%). Based on the results of our experimental data and literature reviews, Perilla essential oil can represent special interest and can be used as an important natural substance for obtaining medicinal and preventive products with the several biological activities.

[Alkaloids of Vinca rosea L. introduced to Western Georgia].

Vinca roseae L. (Саtharanthus rosea (L.) G. Don) was introduced at Kobuleti experimental station of medical plants. The object of investigation was the plant material of Vinca roseae L. collected in May, 2005., September, 2006 and October, 2009. Total alkaloids were obtained in accordance with Atta- ur-Rachman method. The variability of the quantitative and qualitative composition of total alkaloids and vincaleikoblastin (VLB) fraction during vegetation was studied. It was established that the maximal content of total alkaloids and VLB fraction of Vinca roseae L. is accumulated in the phase of secondary flowering, hence the collecting of a plant material is recommended to be made during the aforesaid vegetation phase as for this period it is a rather high output of a raw material, alkaloid complex and VLB faction. Alkaloids vinkaleikoblastin, ajmalicine and new epimer tetrahydroalstonine with С3Н-α- orientation were yielded, separated and identified using modern physical-chemical and spectral methods (13С NMR).

[Development of technology for the substance of poly[3-(3,4-dihydroxyphenyl) glyceric acid] from Symphytum asperum].

Comfrey (Symphytum L.) is used to treat bone fractures, tendon injuries, ulcer lesions of gastrointestinal tract. It promotes wound healing, accelerates exudates resorption in lungs and reduces joints' inflammation. In Georgian folk medicine, herbal remedies from comfrey are used to accelerate regeneration processes. Comfrey contains hepatotoxic and carcinogenic pyrrolizidine alkaloids, besides the main active ingredient is poly [3 - (3,4-dihydroxyphenyl) glyceric acid] (PDPGA). The aim of present work was to develop a technology for the substance - poly [3-(3,4dihydroxyphenyl) glyceric acid] (PDPGA) from comfrey stems, free of toxic pyrrolizidine alkaloids. During the investigation the optimal conditions for extraction and purification have been established: on the first stage pyrrolizidine alkaloids were removed from plant material by supercritical extraction; then the crude polysaccharides' fraction was obtained by water extraction (raw materials/extragent ratio was 1:15 at 90oC, the procedure was carried twice for 60 and 90 minutes). The isolation of the final product - PDPGA from crude polysaccharides' fraction was carried out by ultrafiltration on membrane filters. Based on the results of the investigation the technological scheme for the substance has been developed.